| Identification |
|---|
| HMDB Protein ID
| CDBP04545 |
| Secondary Accession Numbers
| Not Available |
| Name
| E3 ubiquitin-protein ligase RFWD3 |
| Description
| Not Available |
| Synonyms
|
- RING finger and WD repeat domain-containing protein 3
- RING finger protein 201
|
| Gene Name
| RFWD3 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in protein binding |
| Specific Function
| E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53 |
| GO Classification
|
| Function |
| ion binding |
| cation binding |
| metal ion binding |
| binding |
| transition metal ion binding |
| zinc ion binding |
| protein binding |
|
| Cellular Location
|
- Nucleus
|
| Pathways
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Chromosome:1 |
| Locus
| 16q23.1 |
| SNPs
| RFWD3 |
| Gene Sequence
|
>2325 bp
ATGGCTCATGAAGCAATGGAATATGATGTTCAGGTGCAGTTAAATCATGCCGAACAACAG
CCAGCTCCTGCTGGCATGGCCAGCAGCCAAGGGGGACCAGCCCTCCTCCAGCCTGTTCCT
GCTGATGTGGTCAGCAGCCAGGGGGTACCATCCATCCTCCAGCCAGCTCCTGCTGAGGTG
ATCAGCAGCCAAGCGACACCACCCCTGCTCCAGCCTGCTCCGCAACTGTCTGTTGACCTG
ACAGAAGTGGAGGTCTTGGGAGAAGACACTGTGGAGAACATCAATCCAAGAACTTCAGAA
CAACATAGGCAGGGATCTGATGGTAATCACACCATCCCAGCATCTTCGTTGCATTCAATG
ACCAACTTCATCAGCGGACTGCAGAGACTTCATGGCATGCTGGAATTCCTGAGACCTTCA
TCTTCAAACCACAGTGTAGGGCCAATGAGAACAAGAAGGAGGGTATCTGCTTCACGGAGG
GCAAGAGCCGGAGGGTCTCAGAGGACAGACAGTGCCAGGTTGAGAGCACCATTGGATGCT
TACTTTCAGGTGAGCAGGACCCAGCCTGACTTGCCAGCTACCACTTATGATTCAGAGACT
AGGAATCCTGTATCTGAAGAGTTGCAGGTGTCTAGTAGTTCTGATTCTGACAGTGACAGC
TCTGCAGAGTATGGAGGGGTTGTTGACCAGGCAGAGGAATCTGGAGCTGTCATTTTAGAA
GAGCAACTAGCAGGTGTCTCAGCAGAGCAAGAAGTTACATGTATCGATGGAGGCAAGACC
CTCCCCAAACAGCCATCTCCCCAGAAGTCTGAGCCTCTGCTACCTTCTGCTTCTATGGAT
GAGGAAGAAGGGGACACTTGTACAATATGTCTGGAACAGTGGACCAATGCTGGGGACCAC
CGGCTCTCAGCATTACGCTGTGGGCATCTCTTTGGGTATAGGTGCATTTCCACGTGGCTT
AAAGGACAAGTACGAAAATGTCCCCAGTGCAACAAGAAAGCCAGGCACAGTGACATTGTC
GTCCTTTATGCCCGAACCCTGAGAGCTTTGGACACTAGTGAACAGGAGCGCATGAAAAGT
TCCCTACTGAAGGAACAGATGCTAAGGAAACAGGCCGAGTTAGAATCAGCACAGTGCCGA
CTCCAACTGCAGGTCCTCACTGATAAGTGCACTAGGCTTCAAAGGCGTGTTCAGGACTTG
CAAAAACTTACGTCACATCAAAGTCAGAATTTACAGCAACCCAGGGGCTCCCAAGCATGG
GTCCTGAGCTGCTCACCCTCCAGCCAGGGCCAGCACAAGCACAAGTACCACTTCCAAAAG
ACCTTCACAGTATCTCAGGCAGGAAACTGCCGGATCATGGCATACTGTGATGCTCTGAGC
TGCCTGGTGATATCACAGCCTTCTCCTCAGGCCTCTTTTCTTCCAGGCTTTGGTGTTAAG
ATGTTGAGTACTGCCAACATGAAGAGCAGTCAGTACATTCCGATGCATGGCAAACAGATC
CGTGGACTGGCGTTTAGCAGTTACCTCAGAGGCTTGCTACTCTCTGCTTCCCTAGACAAC
ACTATTAAACTGACCAGCCTGGAGACAAATACCGTGGTCCAGACTTATAATGCTGGACGT
CCTGTCTGGAGCTGTTGCTGGTGTCTTGATGAGGCTAACTACATCTATGCTGGACTGGCC
AATGGTTCAATTCTGGTATATGACGTGCGAAACACGAGCAGTCATGTGCAGGAGTTAGTA
GCTCAGAAAGCCAGATGCCCACTGGTCTCCCTGTCATACATGCCCAGAGCTGCCTCAGCT
GCATTTCCATATGGTGGGGTGCTGGCTGGAACCTTGGAGGATGCTTCATTCTGGGAACAG
AAAATGGACTTTTCTCATTGGCCTCATGTGCTGCCCTTGGAGCCAGGGGGCTGCATAGAC
TTTCAGACAGAGAACAGCTCCCGGCACTGTCTTGTGACCTACAGGCCTGATAAAAATCAC
ACCACCATACGAAGTGTGCTGATGGAAATGTCCTACCGACTGGATGACACTGGAAATCCA
ATCTGCTCCTGCCAGCCTGTACATACATTTTTTGGAGGACCTACTTGCAAACTATTGACC
AAAAATGCCATTTTCCAAAGCCCAGAGAATGATGGCAACATCCTGGTGTGTACTGGGGAT
GAAGCAGCAAATTCTGCCCTGCTGTGGGATGCTGCCAGTGGCTCGTTGCTCCAGGACCTA
CAGACCGATCAGCCTGTGTTGGACATCTGCCCATTTGAGGTGAACCGTAACAGCTACTTG
GCTACCTTAACAGAGAAGATGGTCCACATCTATAAGTGGGAGTGA
|
| Protein Properties |
|---|
| Number of Residues
| 774 |
| Molecular Weight
| 85093.1 |
| Theoretical pI
| 6.43 |
| Pfam Domain Function
|
|
| Signals
|
|
|
Transmembrane Regions
|
|
| Protein Sequence
|
>E3 ubiquitin-protein ligase RFWD3
MAHEAMEYDVQVQLNHAEQQPAPAGMASSQGGPALLQPVPADVVSSQGVPSILQPAPAEV
ISSQATPPLLQPAPQLSVDLTEVEVLGEDTVENINPRTSEQHRQGSDGNHTIPASSLHSM
TNFISGLQRLHGMLEFLRPSSSNHSVGPMRTRRRVSASRRARAGGSQRTDSARLRAPLDA
YFQVSRTQPDLPATTYDSETRNPVSEELQVSSSSDSDSDSSAEYGGVVDQAEESGAVILE
EQLAGVSAEQEVTCIDGGKTLPKQPSPQKSEPLLPSASMDEEEGDTCTICLEQWTNAGDH
RLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKS
SLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAW
VLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVK
MLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGR
PVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASA
AFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNH
TTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGD
EAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE
|
| External Links |
|---|
| GenBank ID Protein
| 71143112 |
| UniProtKB/Swiss-Prot ID
| Q6PCD5 |
| UniProtKB/Swiss-Prot Entry Name
| RFWD3_HUMAN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| NM_018124.3 |
| GeneCard ID
| RFWD3 |
| GenAtlas ID
| RFWD3 |
| HGNC ID
| HGNC:25539 |
| References |
|---|
| General References
| Not Available |