Cannabis Compound Database: Showing Protein E3 ubiquitin-protein ligase RNF8 (CDBP04457)

Identification Biological properties Gene properties Protein properties External links References XML Show 3 compounds

Identification

HMDB Protein ID

CDBP04457

Secondary Accession Numbers

Not Available

Name

E3 ubiquitin-protein ligase RNF8

Description

Not Available

Synonyms

RING finger protein 8

Gene Name

RNF8

Protein Type

Enzyme

Biological Properties

General Function

Involved in acid-amino acid ligase activity

Specific Function

E3 ubiquitin-protein ligase required for assembly of repair proteins to sites of DNA damage. Catalyzes the 'Lys-63'- linked ubiquitination of histone H2A and H2AX. Following DNA double-strand breaks (DSBs), it is recruited to the sites of damage by ATM-phosphorylated MDC1, mediates the ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBE2N/UBC13. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation. Enforces the G2/M DNA damage checkpoint. Controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites following DNA double-strand breaks (DSBs). Ubiquitination of histone H2A requires UBE2N but not MMS2 (UBE2V2). May also ubiquitinate histone H2B. Catalyzes the 'Lys- 63'-linked ubiquitination of PCNA. May be required for proper exit from mitosis after spindle checkpoint activation and may regulate cytokinesis. May play a role in the regulation of RXRA-mediated transcriptional activity. Not involved in RXRA ubiquitination by UBE2E2

GO Classification

Component
organelle
membrane-bounded organelle
intracellular membrane-bounded organelle
nucleus
Function
catalytic activity
transition metal ion binding
zinc ion binding
ligase activity
protein binding
ligase activity, forming carbon-nitrogen bonds
acid-amino acid ligase activity
ion binding
cation binding
metal ion binding
binding
Process
macromolecule modification
protein modification process
cellular response to stress
response to dna damage stimulus
response to stimulus
response to stress
metabolic process
macromolecule metabolic process
cellular process
protein modification by small protein conjugation or removal
protein modification by small protein conjugation
cell division
protein ubiquitination

Cellular Location

Nucleus
Midbody

Pathways

Not Available

Gene Properties

Chromosome Location

Chromosome:6

Locus

6p21.3

SNPs

RNF8

Gene Sequence

>1458 bp
ATGGGGGAGCCCGGCTTCTTCGTCACAGGAGACCGCGCCGGTGGCCGGAGCTGGTGCCTG
CGGCGGGTGGGGATGAGCGCCGGGTGGCTGCTGCTGGAAGATGGGTGCGAGGTGACTGTA
GGACGAGGATTTGGTGTCACATACCAACTGGTATCAAAAATCTGCCCCCTGATGATTTCT
CGAAACCACTGTGTTTTGAAGCAGAATCCTGAGGGCCAATGGACAATTATGGACAACAAG
AGTCTAAATGGTGTTTGGCTGAACAGAGCGCGTCTGGAACCTTTAAGGGTCTATTCCATT
CATCAGGGAGACTACATCCAACTTGGAGTGCCTCTGGAAAATAAGGAGAATGCGGAGTAT
GAATATGAAGTTACTGAAGAAGACTGGGAGACAATATATCCTTGTCTTTCCCCAAAGAAT
GACCAAATGATAGAAAAAAATAAGGAATTGAGAACTAAAAGGAAATTCAGTTTGGATGAA
TTAGCAGGTCCTGGAGCTGAAGGCCCCTCAAATTTGAAATCCAAAATAAATAAAGTGTCT
TGTGAATCTGGTCAGCCAGTGAAATCACAGGGGAAAGGTGAAGTGGCCAGTACACCCTCT
GACAATTTGGATCCTAAGTTGACTGCCCTTGAGCCAAGTAAGACCACAGGGGCTCCCATT
TACCCTGGCTTCCCCAAAGTCACAGAGGTTCATCATGAGCAGAAAGCCTCAAACTCTTCA
GCATCTCAGAGAAGCTTACAGATGTTTAAGGTGACCATGTCCAGGATTCTGAGGCTCAAA
ATACAGATGCAGGAAAAACATGAAGCCGTTATGAATGTGAAAAAGCAGACCCAAAAGGGG
AACTCAAAGAAAGTTGTGCAAATGGAGCAGGAACTTCAGGACTTACAGTCCCAGCTGTGT
GCAGAGCAGGCTCAGCAGCAGGCAAGAGTGGAGCAACTAGAGAAGACTTTCCAGGAAGAG
GAACAGCATCTTCAGGGTTTGGAGATAGCCCAAGGAGAAAAGGACCTGAAGCAACAGCTG
GCCCAGGCTCTGCAGGAGCATTGGGCTCTAATGGAAGAGCTAAATCGCAGCAAGAAGGAC
TTTGAAGCAATCATTCAAGCCAAGAACAAAGAATTAGAGCAGACCAAGGAAGAGAAGGAG
AAGATGCAAGCACAGAAGGAAGAAGTTCTTAGCCACATGAATGATGTGCTAGAGAATGAG
CTCCAATGTATTATTTGTTCAGAATACTTCATTGAGGCTGTCACCTTGAACTGTGCCCAC
AGTTTCTGCTCCTACTGTATCAATGAATGGATGAAGCGGAAGATAGAATGCCCCATTTGT
CGGAAGGACATTAAGTCCAAAACGTACTCTTTGGTTCTGGACAATTGCATTAATAAGATG
GTAAATAATCTGAGCTCAGAAGTGAAAGAACGACGAATTGTTCTCATTAGGGAACGAAAA
GCAAAGAGATTGTTCTGA

Protein Properties

Number of Residues

485

Molecular Weight

55517.2

Theoretical pI

7.37

Pfam Domain Function

zf-C3HC4 (PF00097 )
FHA (PF00498 )

Signals

["None"]

Transmembrane Regions

["None"]

Protein Sequence

>E3 ubiquitin-protein ligase RNF8
MGEPGFFVTGDRAGGRSWCLRRVGMSAGWLLLEDGCEVTVGRGFGVTYQLVSKICPLMIS
RNHCVLKQNPEGQWTIMDNKSLNGVWLNRARLEPLRVYSIHQGDYIQLGVPLENKENAEY
EYEVTEEDWETIYPCLSPKNDQMIEKNKELRTKRKFSLDELAGPGAEGPSNLKSKINKVS
CESGQPVKSQGKGEVASTPSDNLDPKLTALEPSKTTGAPIYPGFPKVTEVHHEQKASNSS
ASQRSLQMFKVTMSRILRLKIQMQEKHEAVMNVKKQTQKGNSKKVVQMEQELQDLQSQLC
AEQAQQQARVEQLEKTFQEEEQHLQGLEIAQGEKDLKQQLAQALQEHWALMEELNRSKKD
FEAIIQAKNKELEQTKEEKEKMQAQKEEVLSHMNDVLENELQCIICSEYFIEAVTLNCAH
SFCSYCINEWMKRKIECPICRKDIKSKTYSLVLDNCINKMVNNLSSEVKERRIVLIRERK
AKRLF

External Links

GenBank ID Protein

3721580

UniProtKB/Swiss-Prot ID

O76064

UniProtKB/Swiss-Prot Entry Name

RNF8_HUMAN

PDB IDs

Not Available

GenBank Gene ID

AB012770

GeneCard ID