| Identification |
|---|
| HMDB Protein ID
| CDBP02468 |
| Secondary Accession Numbers
| Not Available |
| Name
| 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase |
| Description
| Not Available |
| Synonyms
|
- 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase
- CYPVIIIB1
- Cytochrome P450 8B1
- Sterol 12-alpha-hydroxylase
|
| Gene Name
| CYP8B1 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in monooxygenase activity |
| Specific Function
| Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids.
|
| GO Classification
|
| Biological Process |
| bile acid biosynthetic process |
| xenobiotic metabolic process |
| Cellular Component |
| endoplasmic reticulum membrane |
| integral to membrane |
| Function |
| ion binding |
| cation binding |
| metal ion binding |
| binding |
| catalytic activity |
| transition metal ion binding |
| electron carrier activity |
| iron ion binding |
| monooxygenase activity |
| heme binding |
| oxidoreductase activity |
| Molecular Function |
| oxygen binding |
| iron ion binding |
| heme binding |
| 7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase activity |
| sterol 12-alpha-hydroxylase activity |
| electron carrier activity |
|
| Cellular Location
|
- Endoplasmic reticulum membrane
- Single-pass membrane protein
- Single-pass membrane protein
- Microsome membrane
|
| Pathways
|
| Name | SMPDB/Pathwhiz | KEGG | | Primary bile acid biosynthesis | Not Available |  | | PPAR signaling pathway | Not Available |  | | Bile Acid Biosynthesis |    | | | Congenital Bile Acid Synthesis Defect Type II |    | Not Available | | Congenital Bile Acid Synthesis Defect Type III |    | Not Available |
|
| Gene Properties |
|---|
| Chromosome Location
| 3 |
| Locus
| 3p22.1 |
| SNPs
| CYP8B1 |
| Gene Sequence
|
>1506 bp
ATGGTTCTCTGGGGTCCAGTGCTGGGAGCTCTGCTGGTGGTCATTGCTGGATACCTGTGC
CTGCCAGGGATGCTCCGACAACGCAGGCCATGGGAGCCCCCTCTGGACAAGGGTACCGTG
CCCTGGCTTGGCCATGCCATGGCTTTCCGGAAGAATATGTTTGAATTTCTGAAGCGCATG
AGGACCAAGCATGGGGATGTGTTCACAGTGCAGCTAGGGGGCCAGTACTTCACCTTCGTC
ATGGACCCCCTCTCCTTTGGCCCCATCCTCAAGGACACACAGAGAAAACTAGACTTTGGG
CAATATGCAAAAAAACTGGTGCTGAAGGTATTTGGATACCGTTCAGTGCAAGGGGACCAT
GAGATGATACACTCAGCCAGCACCAAGCATCTGAGGGGGGATGGCTTGAAGGATCTTAAT
GAGACCATGCTGGACAGCCTGTCCTTTGTAATGCTGACGTCCAAAGGCTGGAGTCTGGAT
GCCAGTTGCTGGCATGAGGACAGCCTCTTTCGCTTCTGCTATTACATCTTGTTCACAGCT
GGCTACCTGAGCTTGTTCGGCTACACGAAGGACAAGGAGCAGGACCTGCTACAGGCAGGA
GAGTTATTCATGGAGTTCCGCAAGTTTGACCTTCTTTTCCCAAGGTTTGTCTACTCCCTG
CTGTGGCCCCGGGAGTGGCTAGAAGTGGGCCGACTCCAGCGTCTCTTTCACAAGATGCTC
TCCGTGAGCCACAGCCAGGAGAAGGAGGGCATCAGCAACTGGCTGGGCAACATGCTTCAG
TTTCTGAGGGAGCAGGGGGTACCCTCAGCTATGCAGGACAAGTTCAACTTCATGATGCTC
TGGGCCTCCCAGGGGAACACGGGGCCTACCTCTTTCTGGGCCCTCTTGTACCTCCTGAAG
CACCCAGAAGCTATTCGGGCTGTGAGGGAGGAAGCTACCCAGGTCCTGGGTGAGGCCAGG
CTGGAGACCAAGCAGTCCTTTGCCTTCAAACTCGGTGCCCTGCAACACACCCCAGTTCTA
GACAGCGTGGTGGAGGAGACGCTGCGGCTGAGGGCTGCACCCACCCTCCTCAGGTTGGTT
CATGAAGACTATACCCTGAAGATGTCCAGTGGGCAGGAGTATCTGTTCCGCCATGGAGAC
ATCCTGGCCCTCTTTCCCTACCTCTCAGTGCACATGGACCCTGACATCCACCCTGAGCCC
ACCGTCTTCAAGTACGATCGCTTCCTCAACCCTAATGGCAGCCGGAAAGTGGACTTCTTC
AAGACAGGCAAGAAGATCCACCACTACACCATGCCCTGGGGTTCGGGCGTTTCCATCTGC
CCTGGGAGGTTCTTTGCACTCAGTGAGGTGAAGCTCTTTATCCTGCTTATGGTCACACAC
TTTGACTTAGAGTTGGTGGACCCTGACACACCACTACCCCATGTTGACCCGCAGCGCTGG
GGTTTTGGCACCATGCAGCCCAGCCACGATGTGCGCTTCCGCTACCGCCTGCATCCTACA
GAGTGA
|
| Protein Properties |
|---|
| Number of Residues
| 501 |
| Molecular Weight
| 58068.01 |
| Theoretical pI
| 8.663 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
MVLWGPVLGALLVVIAGYLCLPGMLRQRRPWEPPLDKGTVPWLGHAMAFRKNMFEFLKRM
RTKHGDVFTVQLGGQYFTFVMDPLSFGSILKDTQRKLDFGQYAKKLVLKVFGYRSVQGDH
EMIHSASTKHLRGDGLKDLNETMLDSLSFVMLTSKGWSLDASCWHEDSLFRFCYYILFTA
GYLSLFGYTKDKEQDLLQAGELFMEFRKFDLLFPRFVYSLLWPREWLEVGRLQRLFHKML
SVSHSQEKEGISNWLGNMLQFLREQGVPSAMQDKFNFMMLWASQGNTGPTSFWALLYLLK
HPEAIRAVREEATQVLGEARLETKQSFAFKLGALQHTPVLDSVVEETLRLRAAPTLLRLV
HEDYTLKMSSGQEYLFRHGDILALFPYLSVHMDPDIHPEPTVFKYDRFLNPNGSRKVDFF
KTGKKIHHYTMPWGSGVSICPGRFFALSEVKLFILLMVTHFDLELVDPDTPLPHVDPQRW
GFGTMQPSHDVRFRYRLHPTE
|
| External Links |
|---|
| GenBank ID Protein
| 4406150 |
| UniProtKB/Swiss-Prot ID
| Q9UNU6 |
| UniProtKB/Swiss-Prot Entry Name
| CP8B1_HUMAN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| AF090320 |
| GeneCard ID
| CYP8B1 |
| GenAtlas ID
| CYP8B1 |
| HGNC ID
| HGNC:2653 |
| References |
|---|
| General References
| Not Available |